The posterior longitudinal ligament (PLL) is described as having deep and superficial layers, though recent studies have suggested that there may be three layers. Additional membranous structures have been reported, although there is no consensus as to their presence or morphology. The vertebral canal and dural sac were opened and the spinal nerve roots and spinal cord removed. The anterior dural ligaments were sectioned at their attachment to the PLL and the dura mater freed from the posterior surface of the vertebral bodies. The borders of the PLL were identified and the superficial and deep layers separated. The PLL is a wide band in the cervical region becoming more denticulate inferiorly, the widest parts being attached to the intervertebral discs (IVD) and adjacent vertebral body where the superficial and deep layers could not be separated. A continuous well developed peridural membrane attaching to the pedicles was present anterior to the deep PLL as well as a separate, thin, incomplete layer in 6 of 18 cadavers, covering the posterior surface of the superficial PLL.